ros fluorescent probe dcfh da molecular probe Search Results


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ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Dojindo Labs dcfh da fluorescent probes
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Thermo Fisher 2 7 dichlorofluorescin diacetate dcfh da
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Thermo Fisher carboxy 2 7 dichlorofluorescein
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Jiancheng Inc dcfh da fluorescent probe
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Thermo Fisher fluorescent probe dcfh da
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fluorescent Probe Dcfh Da, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ApexBio dichloro-dihydro-fluorescein diacetate (dcfh-da
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Yuheng Pharmaceutical Co 2,7-dichlorodihydrofluorescein diacetate fluorescent probes d1002
ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. <t>(D–E)</t> <t>DCFH-DA</t> fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Image Search Results


ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. (D–E) DCFH-DA fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Ultra-small iron-based nanoparticles Mitigate rheumatoid arthritis inflammation via macrophage Repolarization and SLC7A11/GPX4-mediated ferroptosis inhibition

doi: 10.1016/j.mtbio.2025.102551

Figure Lengend Snippet: ROS-scavenging and anti-inflammatory effects of Fe 3 O 4 @C. (A) Cytotoxicity assessment of Fe 3 O 4 @C by CCK-8 assay (n = 3). (B–C) Live/dead staining assessment and quantitative analysis of RAW264.7 macrophages treated with Fe 3 O 4 @C; scale bar, 200 μm. (D–E) DCFH-DA fluorescence (green) demonstrated antioxidant capacity and quantitative analysis of ROS scavenging efficiency (n = 3); scale bar, 200 μm. (F) qRT-PCR analysis of macrophage polarization markers (n = 3). (G) Secretion profiles of inflammatory cytokines measured by ELISA (n = 3). ∗( P < 0.05), ∗∗ ( P < 0.01), ∗∗∗( P < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The ability of Fe 3 O 4 @C to scavenge ROS was assessed utilizing the DCFH-DA fluorescent probe (MedChemExpress, USA).

Techniques: CCK-8 Assay, Staining, Fluorescence, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay